Vitrification-based Cryopreservation of Mouse Gastric Tissue Slices — Organoid Culture

Experimental Overview:
Mouse gastric tissue slices were preserved using vitrification-based cryopreservation technology. Post-thaw organoid culture revealed no significant differences between vitrified tissues and fresh controls.

A. Comparison of Organoid Culture (Day 2, Day 4)

• Fresh Tissue (Control): Control group developed intact organoids and the density of viable organoids increased.
• Vitrified Tissue (Vitrified): Organoid growth in vitrified tissues was consistent with that of fresh tissues, indicating no adverse effects from vitrification.

Conclusion:
Vitrified tissues showed similar organoid density, morphology, and growth rate compared to fresh tissues. This suggests that vitrification effectively preserves tissue viability and supports the proliferation of organoids, providing a reliable technical foundation for long-term organoid culture and downstream applications.