Vitrification-based Cryopreservation of Human Lung Cancer Tissue

Experimental Overview:
Human lung cancer tissues derived from clinical samples were preserved using vitrification-based cryopreservation technology. Post-thaw culture successfully generated organoids. The results demonstrated no significant differences in growth rate or cellular activity between vitrified tissues and controls.

A. Comparison of Post-Cryopreservation Cell

(Left: Fresh control group - Control; Right: Vitrification group - Vitrified)

• After digestion (without culture), ciliated cells in vitrified tissues exhibited motility, similar to the fresh control group. This indicates that cellular activity was not negatively affected by the vitrification process.

 
B. Organoid Growth Progression (Day 0, 7)

• Growth Dynamics:
  ➢ Vitrified tissues differentiated into organoids with progressive size and quantity increases over 7 days, demonstrating growth capacity equivalent to fresh controls.
• Post-Passaging Outcomes:
  ➢ Both control and vitrified groups generated abundant cystic organoids after passaging, indicating no adverse effects of vitrification on organoid proliferation or structural stability.

Conclusion:
Vitrification provides a highly effective and reliable preservation solution for human lung cancer tissues. This method ensures stable cellular viability while also allowing organoid growth and passaging capabilities similar to that observed in fresh tissues, making it well-suited for future research and applications.